LabRat |
08-17-2004 11:45 AM |
TOOT TOOT!!
Just tooting my own horn, if anyone is interested in seeing what I did last summer. We had a visiting scientist from Venezuela come to work in our lab for awhile (actually he usually just took notes while I worked :D ), and this paper was the result. All of the images are mine, taken on a couple of different confocal microscopes by me. I'd put the figures alone in, but they are pretty big, so here's a link to the article. The PDF version is best IMHO.
http://authors.elsevier.com/sd/artic...65380604001014
It's basically just describing the developmental progression of synapse formation on a type of neuron (Purkinje) found in the rat cerebellum. PSD-95 is a post-synaptic protein that is very hard to label using antibodies, but with a lot of TLC I can get it to work for me. Synapsin-1 is a pre-synaptic protein that is rather easy to label. I triple labeled a bunch of slices of cerebellum (PSD-95, Syn-1, and PCs) from different postnatal ages of rat to see how these proteins change their orientation around the developing Purkinje cells. Hope this helps you understand the pics, I think they are rather cool, and NO ONE else has ever done this before. (which is why i was able to get it published) I enjoy looking at all of your guy's stuff, thanks, and keep up the good work!
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