TOOT TOOT!!

LabRat · 08-17-2004, 11:45 AM

Just tooting my own horn, if anyone is interested in seeing what I did last summer. We had a visiting scientist from Venezuela come to work in our lab for awhile (actually he usually just took notes while I worked

), and this paper was the result. All of the images are mine, taken on a couple of different confocal microscopes by me. I'd put the figures alone in, but they are pretty big, so here's a link to the article. The PDF version is best IMHO.
http://authors.elsevier.com/sd/artic...65380604001014
It's basically just describing the developmental progression of synapse formation on a type of neuron (Purkinje) found in the rat cerebellum. PSD-95 is a post-synaptic protein that is very hard to label using antibodies, but with a lot of TLC I can get it to work for me. Synapsin-1 is a pre-synaptic protein that is rather easy to label. I triple labeled a bunch of slices of cerebellum (PSD-95, Syn-1, and PCs) from different postnatal ages of rat to see how these proteins change their orientation around the developing Purkinje cells. Hope this helps you understand the pics, I think they are rather cool, and NO ONE else has ever done this before. (which is why i was able to get it published) I enjoy looking at all of your guy's stuff, thanks, and keep up the good work!

glatt · 08-17-2004, 12:11 PM

I tried to take a look at it, but they want $30 from me to see the PDF.

Do you have a favorite image or two you can post at quality images?

xoxoxoBruce · 08-17-2004, 12:25 PM

Yeah LabRat, even in the ghetto they'll give me a taste before I buy.

LabRat · 08-17-2004, 01:51 PM

crap, sorry. i guess i can see it no prob b/c the university has a subscrip to the journal, so we get free acess to all online content as well. my bad. when i find the original figures, i'll post them asap.

08-17-2004, 11:45 AM	#1
LabRat twatfaced two legged bumhole Join Date: Jun 2004 Posts: 3,143	TOOT TOOT!! Just tooting my own horn, if anyone is interested in seeing what I did last summer. We had a visiting scientist from Venezuela come to work in our lab for awhile (actually he usually just took notes while I worked ), and this paper was the result. All of the images are mine, taken on a couple of different confocal microscopes by me. I'd put the figures alone in, but they are pretty big, so here's a link to the article. The PDF version is best IMHO. http://authors.elsevier.com/sd/artic...65380604001014 It's basically just describing the developmental progression of synapse formation on a type of neuron (Purkinje) found in the rat cerebellum. PSD-95 is a post-synaptic protein that is very hard to label using antibodies, but with a lot of TLC I can get it to work for me. Synapsin-1 is a pre-synaptic protein that is rather easy to label. I triple labeled a bunch of slices of cerebellum (PSD-95, Syn-1, and PCs) from different postnatal ages of rat to see how these proteins change their orientation around the developing Purkinje cells. Hope this helps you understand the pics, I think they are rather cool, and NO ONE else has ever done this before. (which is why i was able to get it published) I enjoy looking at all of your guy's stuff, thanks, and keep up the good work! __________________ Strength does not come from how much weight you can lift, or how many miles you can run. It comes from knowing that you set a goal, and rose to the challenge. Strength comes from within. Last edited by LabRat; 08-17-2004 at 11:47 AM. Reason: spelling, what else??

08-17-2004, 12:25 PM	#3
xoxoxoBruce The future is unwritten Join Date: Oct 2002 Posts: 71,105	Yeah LabRat, even in the ghetto they'll give me a taste before I buy. __________________ The descent of man ~ Nixon, Friedman, Reagan, Trump.

08-17-2004, 01:51 PM	#4
LabRat twatfaced two legged bumhole Join Date: Jun 2004 Posts: 3,143	crap, sorry. i guess i can see it no prob b/c the university has a subscrip to the journal, so we get free acess to all online content as well. my bad. when i find the original figures, i'll post them asap. __________________ Strength does not come from how much weight you can lift, or how many miles you can run. It comes from knowing that you set a goal, and rose to the challenge. Strength comes from within.

08-17-2004, 12:11 PM	#2
glatt ™ Join Date: Jul 2003 Location: Arlington, VA Posts: 27,717	I tried to take a look at it, but they want $30 from me to see the PDF. Do you have a favorite image or two you can post at quality images?

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